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we have examined the life cycle of the organism in detail in an effort to determine whether unique antigens were being expressed that could provide other characteristics distinguishing P. aeruginosa from other organisms. We believe that the examination of the specific P. aeruginosa proteins has not provided an explanation for the unique characteristics of the organism. It is further suggested that the uniqueness of the biochemical makeup of P. aeruginosa or the uniqueness of its life cycle as a pathogen should not be the cause for the risk of P. aeruginosa as a contaminant of the biotherapeutic process. The unique genetic make-up of P. aeruginosa does not provide an explanation for the unique characteristics of the organism as a contaminant of the biotherapeutic process or as a cause for the risk of P. aeruginosa as a contaminant.

As a result of the inability to explain the uniqueness of the organism’s characteristics through its chemical makeup or life cycle, a means of identifying the source of the contaminants is warranted. Researchers have investigated the need for inoculum washing. However, inoculum washing is a traditional part of the plasmid transformation method and is traditionally done prior to the addition of the DNA of interest. Such a procedure was also suggested to aid in reducing the presence of host cells contaminated with plasmid DNA [21]. The authors of the study hypothesized that the washing of the inoculum prior to transformation would remove host cells that might be carrying the plasmid DNA. Although this concept is logical, the gene-carrying plasmid was found in the majority of the isolates. The authors postulate that the contaminant was present in the DNA at the time the inoculum was transformed. However, it is possible that the contaminant was carried as a carrier within a host cell at a low frequency that was able to survive the inoculum washing procedure. Whether that process was viable was not investigated. Another study recently published by the same group suggested that when the target organism could be cultured using a defined-media protocol, there would be no benefit of inoculum washing [22]. That study investigated P

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A smart health IT initiative is being launched to help more patients with vision issues successfully manage their vision therapy across the country.

Vision Care Australia (VCA) today announced it has agreed to share vision therapy data from clinics that have joined a vision therapy data standard known as VISION-01 with a research team to help better understand the benefits of vision therapy.

The VISION-01 vision therapy data standard was designed to improve care for patients with vision problems by standardising the quality of data recording, and to assist in the collection of structured information about the visual function of a patient.

Vision Care Australia Chief Executive Simone Lusardi said the vision therapy data standard data would enable VCA to provide better support to its more than 40,000 vision therapy clients.

“By providing our clients with the highest quality support possible, we are providing them with a better experience in the clinic, and we are partnering with leading vision experts to better understand how vision therapy is beneficial to their health,” Ms Lusardi said.

In Australia, there are now 12 registered vision clinics, or vision therapists, using the VISION-01 data standard, including three in Victoria and NSW. The VISION-01 data standard is supported by the government as part of National Quality Measurement and Monitoring System (NQMMS), which provides a free and comprehensive vision screening and eye health service data collection for providers and consumers.

The vision therapy data standard, combined with VCA’s experience in managing vision therapy clients, will allow VCA to collect data from other vision providers.

“We are excited to partner with these other vision therapy providers to ensure their treatment and services are supported by the highest quality of data,” Ms Lusardi said.

VCA has been involved in providing vision therapy for more than 30 years and is well known in providing the highest quality of care for patients with a range of vision problems.

“Vision therapy is a vital service, and one that we provide effectively in our clinics across Australia, working to minimise the impact vision issues can have on a person’s life,” Ms Lusardi said.

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