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Anantipakshagovindaru by S. L. Bhyrappa (epoch). Bhyrappa (Born 7th of Nov, 1937,. Kannada novelsÂ .
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Book Tags: free, novels, free download, books, website, Google, K.Establishment of the single-cell-derived monoclonal keratinocytes as a model for studying BK channel expression.
The molecular mechanisms by which chemical stimulation induces hyperpolarization in human primary cultured keratinocytes (keratinocytes) are less well understood than those in postganglionic neurons. We investigated the expression of large-conductance Ca2+-activated K(+) (BK(Ca)) channel in a single-cell-derived monoclonal keratinocyte line, K8-5. Ca2+ influx induces membrane hyperpolarization (7 +/- 1 mV) in K8-5 cells after application of 1 mmol/L CaCl2, which is abolished by the addition of 10 micromol/L Gd3+, a BK(Ca) channel blocker. The BK(Ca) channels in K8-5 cells were sensitive to charybdotoxin (CTX) at concentrations ranging from 10 nmol/L to 100 nmol/L and were blocked by the allosteric modulator iberiotoxin (IbTX) at 1 mumol/L. The K8-5 cells were also sensitive to IBX with an EC50 of 12 pmol/L. Cell-attached recordings revealed that the channels in K8-5 cells were closed after application of IbTX. The delayed rectifier K(+) channels in K8-5 cells were blocked by 4-aminopyridine (100 micromol/L) with a time constant of 62 +/- 11 ms in the presence of 1 mmol/L CaCl2. The BK(Ca) channels in K8-5 cells showed different responses to activators of protein kinase C (PKC), phorbol ester and farnesyl