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The other, more recent, invention is the cloning of the intact human factor VIII gene, as set forth in U.S. Pat. No. 5,641,834 to Gray et al. (1994) and the publication of WO 94/28902. More than 250 mutations which constitute more than 95% of all factor VIII mutations are described in accordance with this invention. These mutations include replacements, insertions, inversions, and deletions. In accordance with one embodiment disclosed in Gray et al., 1994 and in accordance with the present invention, there are provided novel nucleic acid compositions encoding these mutated factor VIII polypeptides. The mutated factor VIII gene sequences include a tandem repeat of an octapeptide sequence coding for the residue at position 1,485 in the A chain of the blood coagulation factor VIII protein. These mutations are novel and their presence alters the affinity and/or activity of the factor VIII polypeptide.Dietary constituents that inhibit rat intestinal transport of sugars by affecting surface membrane phosphorylation.
There is evidence that different dietary constituents may modulate sugar and lipid absorption by affecting the activity of phosphodiesterase, cyclic AMP and/or cyclic GMP producing enzymes. Cyclic nucleotides are thought to be involved in the regulation of sugar transport at the brush-border membrane of the intestinal epithelium and studies have shown that different dietary constituents increase cyclic AMP and/or cyclic GMP in target tissues. In this study, young rats were fed on a diet that was high in the polyunsaturated fatty acid, linolenic acid, and a diet that was high in sucrose. Sucrose feeding reduced sucrose absorption from the intestine in vivo, whereas feeding on a diet high in linolenic acid increased absorption. These effects were not due to differences in sucrose
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