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The influence of angiotensin II (Ang II) receptor antagonists on aldosterone production and steroidogenic gene expression in teleosts was studied in vitro. Trout heart and ovarian follicles were incubated in vitro with [14C] cortisol in the absence (control) or presence of an Ang II receptor antagonist, irbesartan, Ang II, or aldosterone. Ang II stimulated aldosterone production and increased expression of CYP11beta1, CYP11beta2, and CYP11beta2 mRNA in trout heart. However, an Ang II receptor antagonist, irbesartan, significantly decreased the Ang II-induced aldosterone production and the expression of CYP11beta1 and CYP11beta2 mRNA in the trout heart. Ovarian follicles, which are likely to be the site of aldosterone production, expressed CYP11beta2 and CYP11beta1 mRNA, and both mRNA levels were increased following stimulation with Ang II. However, irbesartan inhibited the Ang II-induced CYP11beta2 and CYP11beta1 mRNA levels. This study indicates that Ang II stimulates aldosterone production by an increase in the expression of steroidogenic genes in teleosts. Furthermore, Ang II receptor antagonists may be potentially useful to control aldosterone production in teleosts. have a country where you’re not allowed to spit anywhere you like — or behead someone, or hold your nose and say, ‘I hate you all,’ and believe that you have the freedom to do that — that’s not freedom.”

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