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In vertebrates, the embryonic program and subsequent differentiation of the paraxial mesoderm are regulated by multiple intrinsic cues, including FGFs and hedgehog/BMP family proteins, and extrinsic signals derived from the notochord. To investigate the contribution of one of these factors in mesodermal development, we generated a transgenic mouse carrying the GATA5 (globin transcription factor 5) gene in the early mesoderm of the developing limbs. Ectopic expression of GATA5 leads to an expansion and thickening of the limb bud mesoderm. This is accompanied by an up-regulation of the expression of Shh, Fgf8, Fgf4, and Fgf10, all members of the FGF and hedgehog protein signaling pathways, as well as an induction of the expression of Shh in the notochord. Using electroporation, we show that GATA5-expressing mesodermal cells respond to FGF signaling. Ectopic GATA5 induces Shh and Fgf4 expression in Xenopus, whereas no expression of Fgf8 and Fgf10 can be detected. Our data suggest a novel role for GATA5 in the regulation of both intrinsic and extrinsic signaling pathways during paraxial mesoderm development.It was the calm before the storm, and then the storm: The day after The Edit’s “next” big list was released — and featuring the 20th anniversary of Ace Of Base’s “The Sign” — the old song “The Winner Takes It All” was rekindled.
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