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Liver injury may be caused by free radicals, which induce lipid peroxidation. In this study, we evaluated the value of liver catalase and lipid peroxidation products as indicators of the severity of liver injury in rats with N-nitrosodiethylamine (DEN)-induced chronic liver injury. A total of 30 male Wistar rats were fed a standard diet (control) or DEN (200 mg/kg, orally) for 4 months. Rats were killed, and blood and liver tissue were collected. Results from the liver injury/dysfunction tests showed that levels of serum transaminase and alkaline phosphatase activity were significantly increased in DEN-treated rats compared with control rats. Tissue concentrations of the lipid peroxidation products malondialdehyde, thiobarbituric acid-reactive substance (TBARS), and hydroperoxides were also significantly higher in DEN-treated rats than control rats. No significant differences were observed between liver catalase activity in DEN-treated and control rats. These results demonstrate that a chronic N-nitrosodiethylamine-induced rat model of hepatocellular injury exhibits a consistently high level of circulating and hepatic lipid peroxidation products and indicate that these markers may be useful for quantitating tissue damage caused by oxidative stress.The present invention relates to a device for braking
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